5WUM

Crystal structure of mouse importin-alpha1 bound to S385-phosphorylated NLS of EBNA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2016-05-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	77.910, 90.300, 99.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.065 - 2.000
R-factor	0.1872
Rwork	0.186
R-free	0.21150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ukw
RMSD bond length	0.004
RMSD bond angle	0.630
Data reduction software	MOSFLM
Data scaling software	Aimless (0.5.8)
Phasing software	MOLREP
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.970	41.970	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.059	0.015	0.863
Rmeas	0.065
Rpim	0.026
Total number of observations	288684
Number of reflections	48181
<I/σ(I)>	19
Completeness [%]	99.9	99.1	99.3
Redundancy	6	5.2	4.8
CC(1/2)	0.999	1.000	0.653

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	MES, sodium citrate, DTT