5WUM
Crystal structure of mouse importin-alpha1 bound to S385-phosphorylated NLS of EBNA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2016-05-30 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.910, 90.300, 99.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.065 - 2.000 |
| R-factor | 0.1872 |
| Rwork | 0.186 |
| R-free | 0.21150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ukw |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.630 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.8) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 41.970 | 41.970 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.059 | 0.015 | 0.863 |
| Rmeas | 0.065 | ||
| Rpim | 0.026 | ||
| Total number of observations | 288684 | ||
| Number of reflections | 48181 | ||
| <I/σ(I)> | 19 | ||
| Completeness [%] | 99.9 | 99.1 | 99.3 |
| Redundancy | 6 | 5.2 | 4.8 |
| CC(1/2) | 0.999 | 1.000 | 0.653 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | MES, sodium citrate, DTT |
