5WUF

Structural basis for conductance through TRIC cation channels

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-01-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97876
Spacegroup name	H 3 2
Unit cell lengths	91.312, 91.312, 252.799
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.736 - 2.401
R-factor	0.2416
Rwork	0.240
R-free	0.27350
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.424
Data reduction software	XDS
Data scaling software	Aimless (0.5.28)
Phasing software	PHENIX (dev_2481)
Refinement software	PHENIX (dev_2481)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.660	45.660	2.490
High resolution limit [Å]	2.400	8.980	2.400
Rmerge	0.197	0.077	0.454
Number of reflections	30649
<I/σ(I)>	20.8
Completeness [%]	99.8	99.4	98.1
Redundancy	73.6	60.2	40.1
CC(1/2)	1.000	1.000	0.596

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	PEG400 38%, NaCl 100mM, CdCl2 100mM, Tris-HCl, pH 8.5, 100mM