5WUC
Structural basis for conductance through TRIC cation channels
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97876 |
| Spacegroup name | P 63 |
| Unit cell lengths | 64.253, 64.253, 80.811 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.695 - 1.600 |
| R-factor | 0.22 |
| Rwork | 0.219 |
| R-free | 0.23920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.28) |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_2481) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.030 | 46.030 | 1.840 |
| High resolution limit [Å] | 1.600 | 9.000 | 1.800 |
| Rmerge | 0.025 | 0.033 | 0.049 |
| Number of reflections | 48871 | ||
| <I/σ(I)> | 14.5 | ||
| Completeness [%] | 100.0 | 99.4 | 99.4 |
| Redundancy | 20.3 | 21 | 20.4 |
| CC(1/2) | 0.999 | 1.000 | 0.318 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6 | 291 | PEG 200 40%, NaCl 100mM, MgCl2 100mM, MES pH 6.0 100mM |
