5WU6

Crystal structure of apo human Tut1, form IV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2015-12-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 43 21 2
Unit cell lengths	142.530, 142.530, 282.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.993 - 3.209
R-factor	0.2043
Rwork	0.202
R-free	0.24180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wu1
RMSD bond length	0.003
RMSD bond angle	0.770
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		3.290
High resolution limit [Å]	3.200	14.350	3.210
Rmerge	0.375	0.050	2.345
Number of reflections	48397
<I/σ(I)>	14.32	57.42	1.88
Completeness [%]	99.9	96.7	98.9
Redundancy	26.4
CC(1/2)	0.995	1.000	0.677

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	Tris pH 7.0, PEG3350, MgCl2.