5WU3

Crystal structure of human Tut1 bound with MgUTP, form II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2015-06-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	79.794, 85.115, 93.760
Unit cell angles	90.00, 99.56, 90.00

Refinement procedure

Resolution	19.926 - 2.703
R-factor	0.2302
Rwork	0.229
R-free	0.25690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wu2
RMSD bond length	0.005
RMSD bond angle	0.998
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		2.770
High resolution limit [Å]	2.700	12.090	2.700
Rmerge	0.084	0.028	0.760
Number of reflections	33809
<I/σ(I)>	12.27	34.82	1.75
Completeness [%]	99.1	95.6	97
Redundancy	3.4
CC(1/2)	0.997	0.998	0.602

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	Hepes pH 7.5, PEG3350, Tacsimate