5WU2

Crystal structure of human Tut1 bound with BaUTP, form I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2015-05-19
Detector	ADSC QUANTUM 270
Wavelength(s)	1.5
Spacegroup name	P 21 21 21
Unit cell lengths	77.330, 88.400, 184.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.938 - 2.950
R-factor	0.2484
Rwork	0.246
R-free	0.29380
Structure solution method	SIRAS
RMSD bond length	0.003
RMSD bond angle	0.872
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		3.020
High resolution limit [Å]	2.950	13.170	2.950
Rmerge	0.252	0.045	2.102
Number of reflections	51527
<I/σ(I)>	11.26	46.53	1.52
Completeness [%]	99.9	98.3	99.1
Redundancy	28.7
CC(1/2)	0.997	1.000	0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	Hepes pH 7.5, PEG3350, Tacsimate