5WTN

Crystal Structure Analysis of primosome protein DnaB (resiues 1-300) from Geobacillus stearothermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-06
Detector	RAYONIX MX300HE
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	111.356, 118.980, 157.917
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.213 - 2.800
R-factor	0.2297
Rwork	0.228
R-free	0.26250
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.353
Data scaling software	HKL-2000
Phasing software	PHENIX (1.8_1069)
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.800
Rmerge	0.083
Number of reflections	52235
<I/σ(I)>	53.4
Completeness [%]	99.6
Redundancy	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	0.1M Tris (pH 8.5), 1.0M Ammonium sulfate