5WTL

Crystal structure of the periplasmic portion of outer membrane protein A (OmpA) from Capnocytophaga gingivalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97931
Spacegroup name	P 21 21 21
Unit cell lengths	73.205, 87.343, 185.458
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.509 - 2.298
R-factor	0.2057
Rwork	0.203
R-free	0.25510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4erh
RMSD bond length	0.016
RMSD bond angle	2.072
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.509	2.340
High resolution limit [Å]	2.298	2.300
Number of reflections	53087
<I/σ(I)>	8.6
Completeness [%]	98.6
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	100 mM MES, pH 6.0, 200 mM Ca(Ac)2, 20% PEG 8000