5WT3
Pyrococcus abyssi methyltransferase PaTrm5a bound by MTA and cognate tRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.99 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 58.069, 212.302, 130.035 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.140 - 3.204 |
| R-factor | 0.2475 |
| Rwork | 0.247 |
| R-free | 0.25640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zzn |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.940 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.079 | 0.174 |
| Number of reflections | 13684 | |
| <I/σ(I)> | 18.9 | 8.5 |
| Completeness [%] | 99.6 | 98 |
| Redundancy | 6.2 | 5.9 |
| CC(1/2) | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 45% MPD, 100 mM MES (pH 6.5), 200 mM NH4OAc |
