5WSX
The crystal structure of SAV606
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.880, 50.100, 125.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.550 - 2.400 |
| R-factor | 0.20728 |
| Rwork | 0.205 |
| R-free | 0.26113 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pfc |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.597 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.520 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.137 | 0.578 |
| Number of reflections | 12859 | |
| <I/σ(I)> | 10.8 | 2.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.5 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 1.0 M Magnesium citrate, 0.1 M imidazole, 1% glycerol |
