5WRS

Crystal Structure of Fam20A in complex with ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	157.202, 157.202, 144.802
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.943 - 2.750
R-factor	0.2042
Rwork	0.203
R-free	0.23760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wrr
RMSD bond length	0.002
RMSD bond angle	0.614
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.750	7.460	2.750
Rmerge	0.168	0.059	1.470
Number of reflections	55133
<I/σ(I)>	9.9
Completeness [%]	99.9	98.5	100
Redundancy	10.7	10.3	10.6
CC(1/2)		0.998	0.788

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291	ammonium sulfate, HEPES, PEG 400