5WRK
Mu2 subunit of the clathrin adaptor complex AP2 in complex with IRS-1 Y608 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-14 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 64 |
| Unit cell lengths | 126.065, 126.065, 73.403 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.800 - 2.620 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bw8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.948 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.680 |
| High resolution limit [Å] | 2.624 | 2.630 |
| Rmerge | 1.433 | |
| Number of reflections | 20035 | |
| <I/σ(I)> | 28.1 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.3 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.4M Na formate, 0.05M NiCl, 0.1M Na acetate pH 6.0 |
