5WR0
Huisgen cycloaddition for PPARg-LBD labeling by soaking method
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.730, 61.210, 118.350 |
| Unit cell angles | 90.00, 102.95, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.850 |
| R-factor | 0.25229 |
| Rwork | 0.250 |
| R-free | 0.29975 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 115.340 |
| High resolution limit [Å] | 2.850 |
| Number of reflections | 12858 |
| <I/σ(I)> | 12.3 |
| Completeness [%] | 83.7 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.1M Tris-HCl PH 7.4, 0.8M sodium citrate |
