5WPI
The virulence-associated protein HsvA from the fire blight pathogen Erwinia amylovora is a polyamine amidinotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 80 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-02-02 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 127.543, 127.543, 92.229 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.030 - 2.300 |
| R-factor | 0.173 |
| Rwork | 0.170 |
| R-free | 0.22600 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.879 |
| Data reduction software | d*TREK |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.030 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.117 | 0.517 |
| Number of reflections | 38767 | |
| <I/σ(I)> | 7.9 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.93 | 5.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2 M MgCl2, 0.1 M HEPES pH 7.5, and 30% (w/v) PEG 400 |
