5WPA

Structure of human SFPQ/PSPC1 heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.954
Spacegroup name	C 1 2 1
Unit cell lengths	89.229, 67.841, 92.204
Unit cell angles	90.00, 95.06, 90.00

Refinement procedure

Resolution	26.190 - 2.290
R-factor	0.221
Rwork	0.219
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sde
RMSD bond length	0.009
RMSD bond angle	1.050
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.200	2.390
High resolution limit [Å]	2.290	2.290
Rmerge	0.039	0.290
Rpim	0.037	0.270
Number of reflections	24463	2534
<I/σ(I)>	12.8	2.5
Completeness [%]	98.6	97
Redundancy	3.7	3.7
CC(1/2)	0.998	0.954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25% PEG3350, 0.05 M MES, pH 5.5, 0.2 M sodium chloride, 3% aminohexanoic acid