5WOS
Structural and functional insights into Canarypox Virus CNP058 regulation of apoptosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.786, 34.670, 71.726 |
| Unit cell angles | 90.00, 114.92, 90.00 |
Refinement procedure
| Resolution | 33.459 - 2.450 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.24520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tzp |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.543 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.460 | 2.550 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.097 | 0.716 |
| Rpim | 0.084 | 0.710 |
| Number of reflections | 6021 | 703 |
| <I/σ(I)> | 6.1 | 1.1 |
| Completeness [%] | 97.2 | 98.4 |
| Redundancy | 2.7 | 2.7 |
| CC(1/2) | 0.994 | 0.455 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | 0.2 M calcium chloride dihydrate, 0.1 M MES pH 6.0, 20% w/v PEG6000 |
