5WOR
Corkscrew assembly of SOD1 residues 28-38 with familial mutation G37R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 59.615, 59.615, 98.927 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.770 - 2.770 |
| R-factor | 0.214 |
| Rwork | 0.211 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Four strands composed of residues VKVWGSI of PDBID 5DLI were used as initial model |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.800 | 2.900 |
| High resolution limit [Å] | 2.770 | 2.800 |
| Rmerge | 0.183 | 0.907 |
| Number of reflections | 5444 | |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 98.9 | |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | reservoir contained 15% PEG 3350, 0.3M Sodium Malonate |
