5WOA

Crystal Structure of Transient Receptor Potential (TRP) channel TRPV6* in the presence of Gadolinium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.71
Spacegroup name	P 4 21 2
Unit cell lengths	146.077, 146.077, 116.007
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.182 - 3.899
R-factor	0.2733
Rwork	0.271
R-free	0.31590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5iwp
RMSD bond length	0.003
RMSD bond angle	0.558
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.850
Number of reflections	20034
<I/σ(I)>	1.1
Completeness [%]	97.8
Redundancy	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	150 mM Nacl, 100 mM TRIS, 22% PEG 350