5WOA
Crystal Structure of Transient Receptor Potential (TRP) channel TRPV6* in the presence of Gadolinium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.71 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 146.077, 146.077, 116.007 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.182 - 3.899 |
| R-factor | 0.2733 |
| Rwork | 0.271 |
| R-free | 0.31590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5iwp |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.558 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.850 |
| Number of reflections | 20034 |
| <I/σ(I)> | 1.1 |
| Completeness [%] | 97.8 |
| Redundancy | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 150 mM Nacl, 100 mM TRIS, 22% PEG 350 |
