5WO6
Crystal Structure of Transient Receptor Potential (TRP) channel TRPV6cryst
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 145.621, 145.621, 114.136 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.915 - 3.310 |
| R-factor | 0.2888 |
| Rwork | 0.287 |
| R-free | 0.31430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5iwp |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.594 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.300 |
| Number of reflections | 17758 |
| <I/σ(I)> | 1.1 |
| Completeness [%] | 99.8 |
| Redundancy | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 150mM NaCl, 100mM TRIS, 22% PEG 350 |
