5WNB
Structure of antibody 3D3 bound to the linear epitope of RSV G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.11503 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.760, 105.430, 121.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.380 - 2.400 |
| R-factor | 0.2263 |
| Rwork | 0.224 |
| R-free | 0.26820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5i30 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.883 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.380 | 48.380 | 2.380 |
| High resolution limit [Å] | 2.300 | 8.910 | 2.300 |
| Rmerge | 0.130 | 0.050 | 1.154 |
| Rmeas | 0.138 | 0.053 | 1.225 |
| Rpim | 0.045 | 0.017 | 0.406 |
| Total number of observations | 368399 | 7247 | 34482 |
| Number of reflections | 40103 | 793 | 3866 |
| <I/σ(I)> | 13.4 | 32 | 2.7 |
| Completeness [%] | 99.9 | 99.4 | 100 |
| Redundancy | 9.2 | 9.1 | 8.9 |
| CC(1/2) | 0.997 | 0.998 | 0.749 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277.15 | 23% PEG 3350, 0.05 M zinc acetate |
