5WKZ

VH1-69 germline antibody predicted from CR6261

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	77.2
Detector technology	PIXEL
Collection date	2013-02-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03318
Spacegroup name	P 42 21 2
Unit cell lengths	84.233, 84.233, 177.544
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.424 - 1.850
R-factor	0.206
Rwork	0.205
R-free	0.23210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.908
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.850
Number of reflections	55408
<I/σ(I)>	11.3
Completeness [%]	99.7
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	1.6M ammonium sulfate 0.08 M sodium acetate pH 4.60 20% glycerol