5WKY

Bromide sites in the structure of an acid sensing ion channel in a resting state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9191
Spacegroup name	P 21 21 21
Unit cell lengths	109.550, 134.490, 159.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.150 - 4.000
R-factor	0.2817
Rwork	0.281
R-free	0.29890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wku
RMSD bond length	0.004
RMSD bond angle	0.706
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((dev_2597: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.000
High resolution limit [Å]	4.000
Number of reflections	38333
<I/σ(I)>	9.46
Completeness [%]	99.9
Redundancy	7
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	100 mM Tris pH 8.5, 100 mM NaCl, 29% PEG 400, 5 mM BaCl2. Crystal soaked in 150 mM NaBr prior to freezing.