5WKT

3.2-Angstrom In situ Mylar structure of bovine opsin at 100 K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	242.043, 242.043, 110.637
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.740 - 3.200
R-factor	0.2746
Rwork	0.274
R-free	0.29460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j4q
RMSD bond length	0.007
RMSD bond angle	0.875
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.740
High resolution limit [Å]	3.200
Rmerge	0.075
Number of reflections	20539
<I/σ(I)>	9.23
Completeness [%]	100.0
Redundancy	2
CC(1/2)	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	280	100 mM sodium acetate , 3.8 M (NH4)2SO4, ~12% (w/v) trehalose