5WKO

Crystal structure of antibody 27F3 recognizing the HA from A/California/04/2009 (H1N1) influenza virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	77.2
Detector technology	PIXEL
Collection date	2014-05-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 2 2 21
Unit cell lengths	190.580, 191.486, 391.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.451 - 3.492
R-factor	0.2473
Rwork	0.246
R-free	0.26830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4M4Y 4FQH 4FQL
RMSD bond length	0.018
RMSD bond angle	1.588
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.560
High resolution limit [Å]	3.500	3.500
Rpim	0.080	0.450
Number of reflections	177944	8765
<I/σ(I)>	8.7	1.3
Completeness [%]	97.0	94.4
Redundancy	5.7	5
CC(1/2)	0.970	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	1.6 M ammonium sulfate, 0.1 M citric acid pH 4.0