5WKN
Crystal structure of the parainfluenza virus 5 nucleoprotein-phosphoprotein complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-30 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 79.087, 81.063, 152.977 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.180 - 2.653 |
| R-factor | 0.2533 |
| Rwork | 0.251 |
| R-free | 0.28790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4co6 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.742 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MrBUMP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.079 | |
| Rpim | 0.031 | 0.462 |
| Number of reflections | 29196 | 1433 |
| <I/σ(I)> | 30.3 | 1.78 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.3 | 7.5 |
| CC(1/2) | 0.758 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.2 M CaCl2, 0.1 M HEPES sodium pH 7.0 and 24 % (v/v) PEG 400 |
