5WKD
Crystal structure of the segment, GNNQGSN, from the low complexity domain of TDP-43, residues 300-306
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 50.347, 4.777, 14.746 |
| Unit cell angles | 90.00, 101.73, 90.00 |
Refinement procedure
| Resolution | 24.650 - 1.800 |
| R-factor | 0.1843 |
| Rwork | 0.184 |
| R-free | 0.19490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | idealized beta strand |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.804 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.158 | 0.075 | 0.250 |
| Total number of observations | 845 | ||
| Number of reflections | 373 | 45 | 28 |
| <I/σ(I)> | 5.4 | ||
| Completeness [%] | 91.6 | 90 | 84.8 |
| Redundancy | 2.3 | 2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100mM Bis-Tris propane, pH 7.5, 200 mM sodium sulfate, 20% PEG3350 |
