5WJC
Crystal structure of Schizosaccharomyces pombe Mis16 in complex with Eic1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2016-06-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 154.591, 154.591, 52.744 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.886 - 2.298 |
| R-factor | 0.1758 |
| Rwork | 0.172 |
| R-free | 0.21980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xyh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.931 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.097 | 0.760 |
| Rpim | 0.035 | 0.390 |
| Number of reflections | 26459 | 1698 |
| <I/σ(I)> | 22.4 | 1.6 |
| Completeness [%] | 90.8 | 59.7 |
| Redundancy | 8 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296.15 | 100 mM Tris-HCl pH 8.5, 25% PEG 2000MME |
