5WJ2

Crystal structure of the green fluorescent protein Clover

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-17
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.98
Spacegroup name	I 2 2 2
Unit cell lengths	53.063, 135.176, 168.589
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.147 - 2.409
R-factor	0.1943
Rwork	0.193
R-free	0.21720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qyq
RMSD bond length	0.007
RMSD bond angle	0.931
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.116	0.049	0.843
Rmeas	0.124	0.053	0.907
Rpim	0.045	0.020	0.333
Number of reflections	23899
<I/σ(I)>	10.1
Completeness [%]	100.0	99.8	100
Redundancy	7.4	6.8	7.4
CC(1/2)		0.998	0.847

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293	100mM Tris, pH 8.5, 50mM MgCl2, 25% PEG3350