5WIA

Crystal structure of the segment, GNNSYS, from the low complexity domain of TDP-43, residues 370-375

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	4.786, 15.617, 40.679
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.340 - 1.002
R-factor	0.1574
Rwork	0.155
R-free	0.18100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ideal 5-residue polyalanine beta-strand
RMSD bond length	0.005
RMSD bond angle	1.183
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	1.040
High resolution limit [Å]	1.000	2.150	1.000
Rmerge	0.155	0.060	0.554
Rmeas	0.172	0.067	0.714
Rpim	0.072	0.029	0.443
Total number of observations	10032
Number of reflections	1864		111
<I/σ(I)>	5.2
Completeness [%]	95.2	99.6	62.7
Redundancy	5.4	5.3	1.9
CC(1/2)		0.997	0.368

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	100mM bis tris propane 8.5, 200mM sodium nitrate, 20% PEG 3350