5WHW

Using sound pulses to solve the crystal harvesting bottleneck

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97908
Spacegroup name	P 43 21 2
Unit cell lengths	67.719, 67.719, 107.323
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.270 - 1.710
R-factor	0.15268
Rwork	0.152
R-free	0.17332
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fon
RMSD bond length	0.024
RMSD bond angle	2.102
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	57.270
High resolution limit [Å]	1.710
Number of reflections	26111
<I/σ(I)>	6.8
Completeness [%]	99.8
Redundancy	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	1.6 M sodium nitrate, 0.16 M calcium chloride, 0.2 M bis-tris pH 6.5