5WHJ
Crystal structure of Fab fragment of anti-FcRn antibody DX-2507
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 116.170, 116.170, 79.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.072 - 2.150 |
R-factor | 0.1821 |
Rwork | 0.180 |
R-free | 0.22520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3idx |
RMSD bond length | 0.007 |
RMSD bond angle | 0.838 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2863)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.010 | 2.210 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.057 | 0.589 |
Rmeas | 0.061 | 0.022 |
Total number of observations | 218958 | |
Number of reflections | 29879 | 378 |
<I/σ(I)> | 23.8 | 3.8 |
Completeness [%] | 99.3 | 100 |
Redundancy | 7.3 | 7.4 |
CC(1/2) | 0.999 | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | DX-2507 Fab at 9.5 mg/mL against Morpheus screen condition G5 with 10% PEG 20000, 20% PEG 550 |