5WHI
Crystal Structure of Bcl-2-related protein A1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-16 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.510, 43.090, 43.440 |
Unit cell angles | 90.00, 104.15, 90.00 |
Refinement procedure
Resolution | 30.120 - 1.690 |
R-factor | 0.1973 |
Rwork | 0.195 |
R-free | 0.23340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3i1h |
RMSD bond length | 0.008 |
RMSD bond angle | 0.863 |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.120 | 1.720 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmeas | 0.050 | 1.297 |
Rpim | 0.027 | 0.708 |
Total number of observations | 51640 | 2693 |
Number of reflections | 15715 | |
<I/σ(I)> | 13.6 | 1.1 |
Completeness [%] | 98.1 | 98.9 |
Redundancy | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | PEG2000 |