5WHF
Crystal structure of vimentin coil 1B packed in a high-order filamentous form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-05 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.990, 77.270, 123.390 |
| Unit cell angles | 90.00, 95.03, 90.00 |
Refinement procedure
| Resolution | 40.972 - 2.250 |
| R-factor | 0.2372 |
| Rwork | 0.235 |
| R-free | 0.28170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uf1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.830 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.400 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.054 | 0.189 |
| Number of reflections | 32009 | |
| <I/σ(I)> | 7.25 | 3.5 |
| Completeness [%] | 89.0 | 82 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.1 M Bis-Tris pH 6.5, 0.2 M magnesium acetate tetrahydrate and 10% PEG 8000 |
