5WHB

KRas G12V, bound to GDP and miniprotein 225-11(A30R)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	41.002, 232.043, 47.924
Unit cell angles	90.00, 90.10, 90.00

Refinement procedure

Resolution	116.000 - 2.180
R-factor	0.207
Rwork	0.205
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3GF3GFTT
RMSD bond length	0.002
RMSD bond angle	0.460
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	116.000	2.240
High resolution limit [Å]	2.180	2.180
Rmerge	0.097
Number of reflections	44361
<I/σ(I)>	7.9
Completeness [%]	95.3
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.25M CALCIUM CHLORIDE, 24% PEG 3350