5WGB
Crystal Structure of the Human mitochondrial Cysteine Desulfurase in complex with ISD11 and E. coli ACP1 protein at 2.75A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9875 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 140.841, 140.841, 203.301 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.100 - 2.750 |
| R-factor | 0.2164 |
| Rwork | 0.213 |
| R-free | 0.25530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lvm |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.677 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.100 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 20346 | 2020 |
| <I/σ(I)> | 20.3 | |
| Completeness [%] | 99.6 | 99.95 |
| Redundancy | 10.9 | 11.3 |
| CC(1/2) | 0.998 | 0.788 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 285 | 0.1 M MES pH 6.5 0.3 M Ammonium Acetate 20 mM Calcium Acetate 20 mM CaCl2 19 % isopropanol |
