5WG4
Human GRK2 in complex with Gbetagamma subunits and CCG257284
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-10 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 61.146, 241.443, 214.835 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.956 - 2.310 |
| R-factor | 0.235 |
| Rwork | 0.234 |
| R-free | 0.27860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pnk |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.494 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.480 | 2.390 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Number of reflections | 132268 | 6936 |
| <I/σ(I)> | 10.4 | 1.1 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 2 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 50 mM MES pH 6, 6% PEG 3350, 1 M NaCl |
