5WFX

Structural basis for the interaction of 14-3-3beta with Tricarboxylic Acid Cycle intermediate Malate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97929
Spacegroup name	P 21 21 21
Unit cell lengths	59.930, 87.790, 124.044
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.687 - 1.651
R-factor	0.2016
Rwork	0.200
R-free	0.23080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wfu
RMSD bond length	0.006
RMSD bond angle	0.804
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.650
Rmerge	0.060
Number of reflections	78578
<I/σ(I)>	36.3
Completeness [%]	99.3
Redundancy	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	291	2.2M Sodium Malonate pH7.0