5WFU
Structural basis for the interaction of 14-3-3beta with Tricarboxylic Acid Cycle intermediate Malate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-02-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 256.392, 85.604, 56.066 |
| Unit cell angles | 90.00, 101.73, 90.00 |
Refinement procedure
| Resolution | 46.960 - 1.970 |
| R-factor | 0.19157 |
| Rwork | 0.190 |
| R-free | 0.22435 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.874 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.970 |
| Number of reflections | 83288 |
| <I/σ(I)> | 21.9 |
| Completeness [%] | 99.2 |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 291 | 2.1M DL-Malic Acid pH7.0 |
