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5WFO

Ligand-bound Ras:SOS:Ras complex

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 21-ID-F
Synchrotron siteAPS
Beamline21-ID-F
Temperature [K]100
Detector technologyCCD
Collection date2016-06-10
DetectorMARMOSAIC 300 mm CCD
Wavelength(s)0.98
Spacegroup nameI 4 2 2
Unit cell lengths182.735, 182.735, 177.866
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution48.455 - 1.990
R-factor0.1694
Rwork0.169
R-free0.18830
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4nyi
RMSD bond length0.008
RMSD bond angle1.032
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwarePHENIX
Refinement softwarePHENIX (1.9_1692)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.000
High resolution limit [Å]1.990
Rpim0.0410.264
Number of reflections102185
<I/σ(I)>19.6
Completeness [%]100.0
Redundancy8.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP42930.1 M sodium acetate, 2.0 M sodium formate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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