5WFB
Tepsin tENTH domain 1-136.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2013-10-30 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.870, 84.750, 80.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.821 - 1.382 |
| R-factor | 0.1577 |
| Rwork | 0.156 |
| R-free | 0.18530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | basic alpha helical model |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.051 |
| Data reduction software | MOSFLM |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.821 |
| High resolution limit [Å] | 1.380 |
| Number of reflections | 56820 |
| <I/σ(I)> | 14.6 |
| Completeness [%] | 99.6 |
| Redundancy | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 12% (w/v) PEG3350, 4% (v/v) tacsimate pH 5.0 |
