5WE2

Structural Basis for Telomere Length Regulation by the Shelterin Bridge

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.999931
Spacegroup name	P 21 21 21
Unit cell lengths	83.360, 95.390, 109.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.666 - 2.500
R-factor	0.2127
Rwork	0.209
R-free	0.26260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	initial model from SAD phasing
RMSD bond length	0.011
RMSD bond angle	1.276
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.330	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.087	0.660
Number of reflections	30787
<I/σ(I)>	13.1	2.8
Completeness [%]	99.9	100
Redundancy	7.2	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	16-17% w/v PEG3350, 0.2-0.25 M Potassium citrate tribasic monohydrate, 0.1 M Tris-HCl, pH 8.3, 3% 1,5-Diaminopentane hydrochloride