5WDU
HIV-1 Env BG505 SOSIP.664 H72C-H564C trimer in complex with bNAbs PGT122 Fab, 35O22 Fab and NIH45-46 scFv
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03317 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 361.810, 215.850, 176.610 |
| Unit cell angles | 90.00, 114.03, 90.00 |
Refinement procedure
| Resolution | 39.707 - 7.000 |
| R-factor | 0.3036 |
| Rwork | 0.301 |
| R-free | 0.36280 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.097 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 7.100 |
| High resolution limit [Å] | 7.000 | 7.000 |
| Rpim | 0.080 | 0.330 |
| Number of reflections | 19551 | |
| <I/σ(I)> | 7.2 | 1.7 |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 7.6 | 8.1 |
| CC(1/2) | 0.994 | 0.448 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 8% (w/v) polyethylene glycol 8000, 0.1 M Tris, pH 8.5 |
