5WDS
Choanoflagellate Salpingoeca rosetta Ras with GDP bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.999981 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 67.222, 67.222, 184.679 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.530 - 1.850 |
| R-factor | 0.2178 |
| Rwork | 0.216 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5p21 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.885 |
| Data reduction software | XDS (November 3, 2014) |
| Data scaling software | Aimless (0.5.4) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.530 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.093 | 1.437 |
| Rpim | 0.027 | 0.723 |
| Number of reflections | 37417 | 2254 |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.9 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 20% PEG3350, 100 mM Tris-HCl, pH 9.0, 100 mM calcium chloride |
