5WDO

H-Ras bound to GMP-PNP at 277K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	277
Detector technology	PIXEL
Collection date	2017-03-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.88557
Spacegroup name	H 3 2
Unit cell lengths	89.959, 89.959, 136.766
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.589 - 1.650
R-factor	0.1429
Rwork	0.142
R-free	0.16840
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3k8y
RMSD bond length	0.010
RMSD bond angle	1.129
Data reduction software	XDS (Nov 1, 2016)
Data scaling software	Aimless (0.5.32)
Phasing software	PHENIX (1.11.1_2575)
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.590	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.175	3.183
Rpim	0.040	0.756
Number of reflections	25873	1255
<I/σ(I)>	12.6	1.2
Completeness [%]	100.0	100
Redundancy	19.8	18.6
CC(1/2)	0.999	0.514

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	16% PEG3350, 100 mM calcium acetate, pH 7.5