5WCJ
Crystal Structure of Human Methyltransferase-like protein 13 in complex with SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.978630 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.131, 67.450, 71.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.700 |
| R-factor | 0.1882 |
| Rwork | 0.187 |
| R-free | 0.21250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gjy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.488 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 48.980 | 1.730 |
| High resolution limit [Å] | 1.700 | 9.000 | 1.700 |
| Rmerge | 0.053 | 0.028 | 1.050 |
| Rmeas | 0.058 | 0.031 | 1.139 |
| Rpim | 0.023 | 0.012 | 0.435 |
| Total number of observations | 172955 | 1257 | 9432 |
| Number of reflections | 26945 | ||
| <I/σ(I)> | 17.5 | 46.8 | 1.8 |
| Completeness [%] | 99.0 | 99.1 | 98.6 |
| Redundancy | 6.4 | 5.5 | 6.7 |
| CC(1/2) | 0.999 | 0.999 | 0.719 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 291 | 20% PEG3350, 200mM Ammonium Chloride |
