5WCE
Caulobacter crescentus pol III beta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2016-01-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.855, 59.572, 86.490 |
| Unit cell angles | 90.00, 92.89, 90.00 |
Refinement procedure
| Resolution | 86.380 - 1.900 |
| R-factor | 0.18884 |
| Rwork | 0.187 |
| R-free | 0.22624 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tr8 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.763 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.380 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.037 | 0.342 |
| Rpim | 0.035 | 0.324 |
| Number of reflections | 62720 | 5655 |
| <I/σ(I)> | 19.9 | 2.1 |
| Completeness [%] | 93.0 | 84.4 |
| Redundancy | 1.9 | 1.8 |
| CC(1/2) | 0.817 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 298 | PEG |
