5WBY
Crystal structure of mTOR(deltaN)-mLST8-PRAS40(beta-strand) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 138.926, 163.044, 206.666 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 3.100 |
| R-factor | 0.2339 |
| Rwork | 0.233 |
| R-free | 0.27587 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jsn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.148 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 80.000 | 80.000 | 3.110 |
| High resolution limit [Å] | 3.100 | 6.460 | 3.100 |
| Rmerge | 0.113 | 0.033 | 0.692 |
| Rmeas | 0.131 | 0.038 | 0.798 |
| Rpim | 0.064 | 0.019 | 0.386 |
| Total number of observations | 278987 | ||
| Number of reflections | 71263 | 7126 | |
| <I/σ(I)> | 4.6 | ||
| Completeness [%] | 97.2 | 94.5 | 98.5 |
| Redundancy | 3.9 | 3.9 | 4 |
| CC(1/2) | 0.998 | 0.826 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | tacsimate |
