5WBY

Crystal structure of mTOR(deltaN)-mLST8-PRAS40(beta-strand) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97920
Spacegroup name	P 2 21 21
Unit cell lengths	138.926, 163.044, 206.666
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 3.100
R-factor	0.2339
Rwork	0.233
R-free	0.27587
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jsn
RMSD bond length	0.007
RMSD bond angle	1.148
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.000	80.000	3.110
High resolution limit [Å]	3.100	6.460	3.100
Rmerge	0.113	0.033	0.692
Rmeas	0.131	0.038	0.798
Rpim	0.064	0.019	0.386
Total number of observations	278987
Number of reflections	71263		7126
<I/σ(I)>	4.6
Completeness [%]	97.2	94.5	98.5
Redundancy	3.9	3.9	4
CC(1/2)		0.998	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	tacsimate