5WAI

Crystal Structure of a Suz12-Rbbp4-Jarid2-Aebp2 Heterotetrameric Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	93
Detector technology	PIXEL
Collection date	2016-06-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	95.300, 111.428, 253.254
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.100 - 2.900
R-factor	0.1734
Rwork	0.171
R-free	0.21610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gfc
RMSD bond length	0.010
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.100	2.950
High resolution limit [Å]	2.800	2.900
Rmerge	0.150	2.851
Rpim	0.042	0.828
Number of reflections	67533	2989
<I/σ(I)>	22.4	1.5
Completeness [%]	99.1	99.8
Redundancy	13.1	12.6
CC(1/2)	0.990	0.472

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10% PEG6000, 5%v/v(+/-)-2-Methyl-2,4-pentaneediol (MPD) and 100mM HEPES pH 7.5