5W94

Crystal structure of Scc4 in complex with Scc2n and Ctf19n

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-E
Synchrotron site	APS
Beamline	21-ID-E
Temperature [K]	63
Detector technology	CCD
Collection date	2016-02-28
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97918
Spacegroup name	P 62
Unit cell lengths	172.969, 172.969, 145.095
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.940 - 3.193
R-factor	0.2112
Rwork	0.209
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xdn
RMSD bond length	0.011
RMSD bond angle	1.416
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.940
High resolution limit [Å]	3.190	3.190
Number of reflections	40688
<I/σ(I)>	24.37
Completeness [%]	99.1
Redundancy	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	6	291	8% PEG4000, 80 mM PIPES, pH 6.0