5W93
p130Cas complex with paxillin LD1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-27 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.290, 37.741, 152.502 |
| Unit cell angles | 90.00, 100.94, 90.00 |
Refinement procedure
| Resolution | 29.947 - 2.001 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.23740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3T6G chain B |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 31426 | 2436 |
| <I/σ(I)> | 30.3 | 2.5 |
| Completeness [%] | 97.3 | 77.1 |
| Redundancy | 5.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 291 | The final 5 ul crystallization drop contained 0.5 mM protein and 2 mM peptide. The drop and 500 ul reservoir solution both contained 20 mM HEPES, pH 7.5, and 50 mM KCl |
